High Throughput Sample Preparation for Single Cell RNA Sequencing

Demonstrated Protocol, Last Modified on August 9, 2021, Permalink


Chromium Single Cell Gene Expression solutions enable the generation of dual index libraries to study gene expression profiles, cell surface protein expression, and/or CRISPR screening in million-cell experiments. Single cell samples may be multiplexed with the 10x Genomics 3' CellPlex Kit, which provides a species agnostic sample multiplexing solution through the use of a set of 12 Feature Barcode oligonucleotides each conjugated to a lipid.

This protocol provides guidance for preparing samples for Cell Multiplexing Oligo labeling in a high-throughput, plate based format. High throughput formats enable sample labeling at scales more appropriate for applications such as drug and CRISPR screening. Prior experience working with 96-well plates is highly encouraged for this protocol. Pilot experiments are advised to ensure minimal cell loss at each wash step.