Sequencing Metrics & Base Composition of Single Cell 3' v3.1 Dual Index Libraries

Technical Note, v3.1 Next GEM Chemistry, Last Modified on September 8, 2020, Permalink


The Chromium Next GEM Single Cell 3' v3.1 (dual index) workflow produces sequencing-ready Gene Expression libraries alone or in combination with either Cell Surface Protein or CRISPR Screening libraries from the same single cells. This enables simultaneous profiling of cellular features in combination with gene expression profiling. This Technical Note presents a comparison of sequencing metrics for various Single Cell 3' Dual Index library types across Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.