Technical Note, v3.1 Next GEM Chemistry, Last Modified on March 31, 2021, Permalink
Chromium Next GEM Single Cell 3’ LT v3.1 is a low throughput, cost-effective solution for smaller-scale and pilot studies, which enables profiling whole transcriptome at the single cell level for one hundred to a thousand cells per sample. In combination with Feature Barcode technology, the assay also enables simultaneous cell surface protein detection in single cells. This Technical Note highlights sample preparation, reagents, and workflow specifics for Single Cell 3’ LT v3.1, along with information about cell dilution, cell multiplet rate, signal-to-noise data profile, and cluster defining for low cell loads. A comparison of representative data derived from the low throughput Single Cell 3’ LT v3.1 assay versus the standard Single Cell 3’ v3.1 assay is also presented.