Single Cell Gene Expression HT Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on August 19, 2021, Permalink

Protocol HT HT with CRISPR Screening HT with Cell Surface Protein HT with Cell Multiplexing HT with CRISPR Screening & Cell Multiplexing HT with Cell Surface Protein & Cell Multiplexing
Single Cell Protocols - Cell Preparation Guide
Methanol Fixation of Cells for Single Cell RNA Sequencing -
Isolation of Nuclei for Single Cell RNA Sequencing ✓*
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling ✓* - ✓* ✓* - ✓*
Tumor Dissociation for Single Cell RNA Sequencing - -
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing ✓* ✓* ✓* ✓* ✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing - - - - - -
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing - -
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing - -
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols - - -
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing ✓* ✓* ✓* ✓* ✓* ✓*
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols - - -
High Throughput Sample Preparation for Single Cell RNA Sequencing - - - - -

✓ Compatible (tested in-house)
✓* Untested but low risk
** Compatible but unsupported
- Untested, use at your own risk
✘ Incompatible