Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing

Demonstrated Protocol, Last Modified on March 31, 2021, Permalink

CG00055_Demonstrated_Protocol_Dissociation_Mouse_Neural_Tissue_Rev C.pdf

20191126_CG00055_Dissociation_Mouse_Neural_Tissue_RevBtoRevC.pdf

This protocol outlines how to obtain a single cell suspension from embryonic mouse brain tissue for use with 10x Genomics Single Cell protocols. The surgical dissection of embryonic mouse tissue is not described here.

This demonstrated protocol is optimized for counting cells in the range of 700-1200 cells/µl. If using the Single Cell 3' LT v3.1 (low throughput) application, ensure cells are counted as indicated in this protocol and then diluted to the LT specific optimal loading concentration of 100-600 cells/µl using the Cell Dilution Overview in the LT User Guide.