Index

Technical Notes

• Assay Scheme and Configuration of Chromium Single Cell 3' v2 Libraries
• Base Composition of Sequencing Reads of Chromium Single Cell 3’ v2 Libraries
• Biological & Technical Variation in Single Cell Gene Expression Experiments
• Chromium Connect: Consistent Automated Single Cell Gene Expression Library Generation
• Chromium Next GEM Single Cell 3' LT v3.1: Reagents, Workflow, & Data Overview
• Chromium Next GEM Single Cell 3ʹ v3.1: Cell Multiplexing
• Chromium Next GEM Single Cell 3ʹ v3.1: Dual Index Libraries
• Chromium Next GEM Single Cell 3ʹ v3.1: Reagent & Workflow Updates
• Chromium Single Cell 3ʹ v3 Reagent, Workflow & Software Updates
• Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® NovaSeq®
• Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® Sequencers
• Chromium Single Cell Applications - Guidelines for Optimal Sample Preparation
• Chromium Single Cell CRISPR Screening – Experimental Planning Guide
• Guide RNA Specifications Compatible with Feature Barcoding technology for CRISPR Screening
• Guidelines for Accurate Target Cell Counts Using 10x Genomics® Single Cell Solutions
• Importance of Cell Stock Concentration for Accurate Target Cell Recovery
• Instructions to Download and Process BAM files of 1.3 Million Brain Cells
• Instructions to Download and Process FASTQs of 1.3M Brain Cells
• Interpreting Cell Ranger Web Summary Files for Single Cell Gene Expression Assays
• Interpreting Intronic and Antisense Reads in 10x Genomics Single Cell Gene Expression Data
• Performance of Blood Collection Tubes and PBMC Isolation Methods for Single Cell Gene Expression
• Quality Control of Cell Surface Protein Labeling using Flow Cytometry
• Removal of Dead Cells from Single Cell Suspensions Improves Performance for 10x Genomics® Single Cell Applications
• Resolving Cell Types as a Function of Read Depth and Cell Number
• Sequencing Metrics & Base Composition of Single Cell 3' v3.1 Dual Index Libraries

Demonstrated Protocols

• Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols
• Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
• Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing
• Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling
• Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
• Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
• Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
• Isolation of Nuclei for Single Cell RNA Sequencing
• Methanol Fixation of Cells for Single Cell RNA Sequencing
• Moss Protoplast Suspension for Single Cell RNA Sequencing
• Nuclei Isolation from Adult Mouse Brain Tissue for Single Cell RNA Sequencing
• Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing
• Single Cell Gene Expression Demonstrated Protocol Compatibility Table
• Single Cell Protocols - Cell Preparation Guide
• Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
• Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing
• Tumor Dissociation for Single Cell RNA Sequencing

Specifications

• Chromium Connect Declaration of Conformity
• Chromium Connect Specifications
• Chromium Controller Specifications
• Chromium Single Cell Controller Specifications
• Sample Index Sets for Single Cell 3'
• Sequencing Requirements for Single Cell 3'
• Sequencing Requirements for Single Cell 3' LT

Instrument Softwares

• Chromium Connect Instrument Software Update Procedure
• Chromium Connect Software Release Note

User Guides

• Chromium Connect Quick Reference Cards
• Chromium Connect User Guide
• Chromium Controller Readiness Test
• Chromium Controller Readiness Test with Chromium Next GEM Test Chip
• Chromium Controller Training Kit
• Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits User Guide (v3.1 Chemistry)
• Chromium Next GEM Single Cell 3' LT Reagent Kits (v3.1 Dual Index) User Guide
• Chromium Next GEM Single Cell 3ʹ LT Reagent Kits (v3.1 Dual Index) with Feature Barcode technology for Cell Surface Protein
• Chromium Next GEM Training Kit User Guide
• Chromium Single Cell 3' Protocol Time Planner (v2 Chemistry)
• Chromium Single Cell 3' Reagent Kits User Guide (v2 Chemistry)
• Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry)
• Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for CRISPR Screening
• Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for Cell Surface Protein
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index)
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening and Cell Multiplexing
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Multiplexing
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Cell Multiplexing
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry)
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening
• Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
• Chromium Single Cell 3' v2 Reagent Kit, Quick Reference Cards (v2 Chemistry)
• Chromium Single Cell Controller Readiness Test
• Selecting the Correct Single Cell Gene Expression LT User Guide
• Selecting the Correct Single Cell Gene Expression User Guide

Firmware Upgrades

• Chromium Controller Firmware Release Note
• Chromium Controller Firmware Update Procedure

Product Notifications

• Chromium Controller Touchscreen Product Defect Notification
• Training Module Single Cell Gene Expression

SDS

• Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits Safety Data Sheets (v3.1 Chemistry)
• Chromium Next GEM Single Cell 3' LT Reagent Kits Safety Data Sheets (LT v3.1 Dual Index Chemistry)
• Chromium Next GEM Single Cell 3' Reagent Kits Safety Data Sheets (v3.1 Chemistry)
• Chromium Next GEM Single Cell 3' Reagent Kits Safety Data Sheets (v3.1 Dual Index Chemistry)
• Chromium Next GEM Training Kit Safety Data Sheets
• Chromium Single Cell 3' Reagent Kits Safety Data Sheets (v2 Chemistry)
• Chromium Single Cell 3' Reagent Kits Safety Data Sheets (v3 Chemistry)
• Chromium Training Kit Safety Data Sheets

Getting Started

• Getting Started: Single Cell 3’ Gene Expression
• Next GEM Technology for Single Cell Gene Expression
• Single Cell Gene Expression LT with Feature Barcoding technology
• Single Cell Gene Expression with Cell Multiplexing technology
• Single Cell Gene Expression with Feature Barcoding technology

Software

• Overview
• System Requirements
• Downloads

Datasets

• Datasets