Chromium Connect: Consistent Automated Single Cell Gene Expression Library Generation

Technical Note, Last Modified on February 24, 2020, Permalink


Chromium Connect automates the preparation of sequencing-ready, single cell libraries from input cell samples, minimizing technical variation in single cell gene expression data. Generation of Chromium Single Cell 3’ Gene Expression libraries on the Chromium Connect instrument includes automated Gel Beads-in-emulsion (GEM) generation, barcoding, and library preparation from single cell suspensions, along with additional functionalities for library quantification and pooling. This Technical Note highlights the consistency and reproducibility of single cell gene expression data derived from libraries generated using the Chromium Connect automated workflow.