Isolation of Nuclei for Single Cell DNA Sequencing

Demonstrated Protocol, Last Modified on July 20, 2018, Permalink


This protocol outlines how to isolate, wash, and count single nuclei from frozen solid tissue samples for use with 10x Genomics Single Cell DNA protocol. Modifications to this protocol (e.g. homogenization, centrifugation speed/time, and filtration steps) may be required when working with different sample types. Refer to Appendix for a practice protocol for nuclei isolation from a cell suspension.