Specifications, Last Modified on November 30, 2020, Permalink
The Chromium Single Cell ATAC Solution produces Illumina® sequencer-ready libraries.
Sequencing requirements of Multiome ATAC libraries require different considerations, refer to the Sequencing Requirements for Single Cell Multiome ATAC + Gene Expression page for specifications.
Recommended Sequencing Depth: 25,000 read pairs per nucleus (50,000 individual reads. 25,000 from R1, 25,000 from R2)
Dual-Indexed Sequencing Run: Single Cell ATAC libraries are dual-indexed. Please note, this is different than most 10x products which are single-indexed.
PhiX Spike-In Recommendations: 1%.
|Read||Read 1||i7 Index||i5 Index||Read 2|
|Purpose||Transposed DNA||Sample Index||10x Barcode||Transposed DNA|
* Do not pool Chromium Single Cell ATAC libraries with other 10x libraries while sequencing
** Can be 1 bp shorter to accommodate NovaSeq v1.0 100 cycle kits