Next GEM Technology for Single Cell ATAC

Getting Started, Last Modified on June 14, 2022, Permalink

Learn more about the Next GEM Reagents and Chips for the Single Cell ATAC Solution

Next GEM is part of 10x Genomics’ commitment to continuous product improvements.

• Next GEM is a technology that is built on a new chip architecture
• Next GEM combines new chips and reagents
• Next GEM reagents are optimized for performance on the Next GEM microfluidic chips

Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 and Next GEM Chip H Single Cell Kits provide the same features and comparable assay performance as Single Cell ATAC Reagent Kits v1 and Chip E Single Cell Kits.

• High sensitivity, low multiplet rates
• High number of fragments per nucleus, with low mitochondrial reads
• Robust workflow
• Simple chip loading
• Compatible with nuclei
• Industry leading nuclei capture rates (~65%)
• Broad range of sample throughput (1-8)
• Broad range of nuclei throughput per sample (500 - 10,000)

User Guide

• Chromium Next GEM Single Cell ATAC Reagent Kits v1.1

Demonstrated Protocols

• Nuclei Isolation for Single Cell ATAC Sequencing
• Nuclei Isolation from Mouse Brain Tissue for Single Cell ATAC Sequencing

Technical Notes

• Application Note: Performance of Chromium Next GEM Single Cell ATAC Reagent Kits
• Chromium Next GEM Single Cell ATAC v1.1 Reagent, Workflow and Software Updates

MSDS

• Chromium Next GEM Single Cell ATAC Library Reagent Kits Safety Data Sheets