Demonstrated Protocol, Last Modified on September 6, 2019, Permalink
CG00039_Demonstrated_Protocol_FreshFrozenHumanPBMCs_RevD.pdf
20190802_CG00039_FreshFrozenHumanPBMCs_RevCtoRevD.pdf
This Demonstrated Protocol outlines (i) how to generate cryopreserved human peripheral blood mononuclear cells (PBMC) and (ii) how to thaw, wash, and count cryopreserved human PBMC in preparation for use in 10x Genomics Single Cell Protocols. While this Demonstrated Protocol is specific to PBMC, the Protocol may be used as a basis for handling other primary cells in preparation for use in 10x Genomics Single Cell Protocols.