Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing

Demonstrated Protocol, Last Modified on December 4, 2019, Permalink

CG000233_Demonstrated_Protocol_Thawing_Dissociated_TumorCells_Rev A.pdf

This protocol outlines thawing of frozen dissociated tumor cells for use with 10x Genomics Single Cell protocols. The protocol was demonstrated using dissociated tumor cells from glioblastoma, renal cell carcinoma, and endometrial cancer patients.

While this Demonstrated Protocol is specific to dissociated tumor cells, the protocol may be used as a basis for handling other primary/fragile cells in preparation for use in the 10x Genomics Single Cell protocols.