The Visium Spatial Gene Expression for FFPE is designed to measure mRNA in tissue sections derived from formalin fixed & paraffin embedded (FFPE) tissue samples and requires a Visium Spatial slide with intact tissue sections as input. Immunostaining tissue sections with fluorescently labeled antibodies enables simultaneous protein detection. This protocol outlines deparaffinization, decrosslinking, immunofluorescence (IF) staining, and imaging of tissue for use with 10x Genomics Visium Spatial Gene Expression for FFPE assay. Deparaffinized, decrosslinked, and stained tissue sections are inputs for the downstream Visium Spatial Gene Expression for FFPE workflow.
Important
This protocol is only compatible with Spatial Gene Expression for FFPE reagent kits. Do NOT use with the Visium assay for snap frozen and OCT embedded tissue.