Sequencing Requirements for Genome & Exome

Specifications, Last Modified on August 11, 2017, Permalink

Genome - Human

Supported Sequencers

• Illumina® NovaSeq
• Illumina® HiSeq X Ten/Five
• Illumina® HiSeq 3000/4000
• Illumina® HiSeq 2500 Rapid Run

Required Sequencing: 128Gb *

Read	Read1	i7 Index	i5 Index	Read2
Length	150	8	0	150

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Exome - Human

Supported Sequencers

• Illumina® HiSeq 3000/4000
• Illumina® HiSeq 2500 Rapid Run

Required Sequencing: 9Gb * (for Agilent SureSelect Human All Exon V6)

Read	Read1	i7 Index	i5 Index	Read2
Length	100	8	0	100

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*Adjust sequencing depth for the required performance or application. For exome/targeted applications, adjust sequencing based on panel size.