Technical Note, v1 Chemistry, Last Modified on November 19, 2018, Permalink
Technical Note - Sample Preparation Recommendations for the Chromium Genome Kit
This Technical Note provides guidance for high molecular weight (HMW) genomic DNA (gDNA) extraction, including general recommendations and links to specific protocols recommended by 10x Genomics. The Chromium Genome workflow generates long-range information across the length of individual DNA molecules. Starting the process with HMW gDNA will typically result in better application performance, such as increased haplotype phase block length and ability to call structural variants. Sample types discussed include cells, blood, fresh-frozen tissue, plant tissue, and insects.