Demonstrated Protocol, Last Modified on June 30, 2017, Permalink
The Chromium™ Genome Protocols generate long-range information across the length of individual DNA molecules. Starting the process with High Molecular Weight (HMW) genomic DNA (gDNA) will typically result in better application performance, such as increased haplotype phase block length and ability to call structural variants. Optimal performance has been characterized on input gDNA with a mean length >50 kb.
This Demonstrated Protocol outlines a method for gDNA extraction from suspension cells. This Protocol can produce gDNA with an average size of >200 kb when analyzed on a pulsed-field gel, and typically >80 kb after the Chromium Genome Protocols. There are many variables that will affect gDNA quality in sample types, including sample age, transportation methods, type of sample, additives, freezing method, etc.
This Protocol outlines gDNA extraction from suspension cells and although not demonstrated here, results are expected to be similar with adherent cells.