Demonstrated Protocol, Last Modified on July 7, 2017, Permalink
The Chromium Genome Protocols generate long-range information across the length of individual DNA molecules. Starting the process with High Molecular Weight (HMW) genomic DNA (gDNA) will typically result in better application performance, such as increased haplotype phase block length and ability to call structural variants. Optimal performance has been characterized on input gDNA with a mean length >50 kb.
This Demonstrated Protocol outlines the recommended method for HMW gDNA extraction from 200 μl fresh whole blood. No additional reagents or consumables are required other than those listed in the Chromium Genome User Guides.