Single-stranded DNA Damage and its Effects on Chromium™ Genome Application Performance

Technical Note, v2 Chemistry, Last Modified on October 11, 2017, Permalink

CG000118_10x_Technical Note_ssDNA Damage_RevA.pdf

Technical Note - Single-stranded DNA Damage and its Effects on Chromium™ Genome Application Performance

This Technical Note demonstrates that single-stranded DNA damage (ssDNA) caused by heat or enzymatic nicking can influence DNA quality and, subsequently, the performance of Chromium Genome Applications. DNA quality is impacted by both the length of the HMW gDNA sample and the extent of damage to the DNA. While DNA length can be assessed by pulsed-field gel electrophoresis, ssDNA damage is difficult to assess. Recommendations for minimizing DNA damage during gDNA extraction and storage are discussed.

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