Technical Note, v2 Chemistry, Last Modified on November 19, 2018, Permalink
Technical Note - Chromium Genome v2 Libraries – Gel Bead Oligo Primer Contamination Affects Sequencing & Application Performance
This Technical Note describes differences in sequencing and application performance as a result of residual Gel Bead oligo primers that may be present in Chromium Genome v2 libraries. Throughout library preparation, SPRIselect beads remove excess Gel Bead oligo primers. Inefficient SPRIselect cleanup can result in residual Gel Bead oligo primers in the final library, decreasing sequencing metrics such as percentage of clusters passing filter and increasing the unmapped read fraction. Additional SPRIselect cleanups, if needed, can be included to remove excess Gel Bead oligo primers from Chromium Genome v2 libraries prior to sequencing.
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