Technical Note, v2 Chemistry, Last Modified on November 19, 2018, Permalink
Technical Note - Chromium Genome Application Performance as a Result of DNA Quality and Sequencing Depth
This Technical Note characterizes the performance of the Chromium Genome Solution as a function of both DNA quality and sequencing depth. The Chromium Genome Protocols generate long-range information across the length of individual DNA molecules. Starting the process with High Molecular Weight genomic DNA (HMW gDNA) will typically result in better application performance, such as increased haplotype phase block length and ability to call structural variants. However, lower quality genomic DNA and lower sequencing depth also provide significant value.