## What is the 10x Genomics Cloud CLI?

The 10x Genomics Cloud CLI is a command line tool that allows you to upload custom references and FASTQ files to your 10x Genomics account, create projects from the command line, and manage other tasks related to your 10x Genomics account.

It is an executable that can be run directly and requires no compilation or installation.

Download the 10x Genomics Cloud CLI for your operating system and unpack it to a convenient location. Make sure you download the 10x Genomics Cloud CLI for the operating system where your data lives. Your home directory is a good default location.

Optionally, install the binary in a location where you can execute it globally (e.g., /usr/local/bin).

#### Linux

If you’re using a Linux system, use the command below to download:

curl -f -o txg-linux-v1.1.1.tar.gz https://cf.10xgenomics.com/cloud-cli/1.1.1/txg-linux-v1.1.1.tar.gz


and then to unpack:

tar -zxvf txg-linux-v1.1.1.tar.gz


#### macOS

If you’re using a macOS system, open the Terminal app and use the command below to download:

curl -f -o txg-macos-v1.1.1.zip https://cf.10xgenomics.com/cloud-cli/1.1.1/txg-macos-v1.1.1.zip


and then to unpack:

unzip txg-macos-v1.1.1.zip


#### Windows

10x Genomics Cloud CLI for Windows v1.1.1

curl -f -o txg-windows-v1.1.1.zip https://cf.10xgenomics.com/cloud-cli/1.1.1/txg-windows-v1.1.1.zip


## Running commands

To run the 10x Genomics Cloud CLI, you need to enter the path to the executable.

Option 1: You can specify the full path to the executable every time you want to run a command. For example:

/home/user.name/apps/txg-linux-v1.1.1/txg help

or for Windows users:
/home/user.name/apps/txg-windows-v1.1.1/txg.exe help


Option 2: Or you can navigate into the executable's directory, for example txg-linux-v1.1.1, and run the command from within the directory every time you want to run a command. For example:

cd txg-linux-v1.1.1/
./txg help


or for Windows users:

cd txg-windows-v1.1.1/
txg.exe help


Option 3: Or you can add the txg executable to your $PATH. This means you can type txg [command] from anywhere to access the executable and run commands. If you use the echo command below, you should see a list of directories separated by colons similar to below. echo$PATH
/usr/local/bin:/usr/bin:/bin:/usr/sbin:/sbin:/usr/local/go/bin


If you put the 10x Genomics Cloud CLI executable in one of these directories, you've added it to your $PATH and can run the txg command from anywhere. There are several ways to add an executable to your$PATH. Here's one method:

cd txg-macos-v1.1.1/

or
cd txg-linux-v1.1.1/


Then print the path to current directory using this command:

pwd


The output should be the directory containing the txg executable. Copy the outputted path and use it in the following export command to add it into the $PATH variable. export PATH=[directory containing the executable]:$PATH


For example:

export PATH=/mnt/home/user.name/yard/apps/txg-linux-v1.1.1:$PATH  To verify you correctly added the 10x Genomics Cloud CLI to your$PATH, enter:

which txg


You should see output similar to the following:

/mnt/home/user.name/yard/apps/txg-linux-v1.1.1/txg


Note: this only temporarily adds the executable to your $PATH. It's only valid in the current shell session. To permanently add txg to your$PATH, add the export command you just used to your .bashrc (Linux) or .zshrc (macOS v10.15+), which is a special script that runs everytime you login to your system. For more details, view this tutorial.

For instructions on adding files to \$PATH on Windows, read more here.

To upload files into a project, use the command provided in your project. You will also need to specify the project ID and the path to the FASTQ files.

The project ID is a unique identifier that is automatically generated when you create a project. You can find the project ID in the upload command provided in your project.

./txg fastqs upload --project-id [insert unique project id] [insert path to fastqs]


Your FASTQ files must follow the Illumina naming convention, ex. SampleName_S1_L001_R1_001.fastq.gz.

Below are three example commands for uploading FASTQ files to a project.

Note: the 10x Genomics Cloud CLI will only upload FASTQ files to your project, even if there are other file types present in the path you provide.

To upload all the FASTQ files in a given folder:

./txg fastqs upload --project-id fo91F3f3jQTipjfGKpdV6lg myfastqs/


./txg fastqs upload --project-id fo91F3f3jQTipjfGKpdV6lg myfastqs/SampleName_S1_L001_R1_001.fastq.gz


To upload all the FASTQ files in a folder that begin with Sample01:

./txg fastqs upload --project-id fo91F3f3jQTipjfGKpdV6lg myfastqs/Sample01*


After verifying your identity, you will see a message similar to the following:

You are about to upload 3 file(s) to the following project
Project ID: 7e8fc23dd-fde3-4138-4138-f18aa5d57a96
Project Name: "My First Project"
Number of files: 3
/Users/your.name/myfastqs/test_sample_S1_L001_I1_001.fastq.gz (1.5 kB)
/Users/your.name/myfastqs/test_sample_S1_L001_R1_001.fastq.gz (2.9 kB)
/Users/your.name/myfastqs/test_sample_S1_L001_R2_001.fastq.gz (7.2 kB)
Total: 12 kB.
To stop the upload, press Ctrl+C.
Proceed? [y/N]

Example success message:

Starting upload...
/Users/your.name/myfastqs/test_sample_S1_L001_I1_001.fastq.gz (1.5 kB) Done
/Users/your.name/myfastqs/test_sample_S1_L001_R1_001.fastq.gz (2.9 kB) Done
/Users/your.name/myfastqs/test_sample_S1_L001_R2_001.fastq.gz (7.2 kB) Done
You can view and analyze your data in the project "My First Project".